產(chǎn)品描述：
FlashGel® System for Recovery
5分鐘DNA回收
產(chǎn)品介紹：
FlashGel® System for Recovery
5分鐘DNA回收
利用回收的FlashGel ®系統(tǒng)直接回收DNA無(wú)需制備瓊脂糖凝膠，帶切除和純化。該系統(tǒng)可提供高回收率，
無(wú)抑制劑和紫外線損害，只需5-10min的簡(jiǎn)單操作。
•從上樣到回收只需5min
•直接從cassette回收樣品，不需切除和純化
•在電泳槽里可視化的樣品回收，不需紫外線照射，80％ - 100％的回收率
快速簡(jiǎn)單的程序
1）在頂行的小孔加入樣品
2）電泳，直到樣品到第二行孔處（2-5min）
3）停止電泳，并加入FlashGel ®回收緩沖液
4）從孔里吸取DNA
該FlashGel ®回收系統(tǒng)無(wú)需切膠和純化。DNA遷移到第二行孔時(shí)，借助FlashGel ®緩沖液通過(guò)吸液管方便
地提取。樣品回收率達(dá)80％ - 100％，擺脫抑制劑和可用于下一步擴(kuò)增，克隆或其他技術(shù)，均不需額外
的純化步驟。
要開始使用回收，需訂購(gòu)FlashGel ®回收試劑盒（Cat. No. 57050）
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貨號(hào)
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描述
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規(guī)格
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57051
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FlashGel ® 回收Cassettes-9/pk
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1.2%agarose, 8+1 double-tier
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57060
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FlashGel ® 回收緩沖液 ready-to-use
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2 x 500ul
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50475
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FlashGel ®QuantLadder 100bp(3ng)-1.5kb(30ng) ready-to-load 50次
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250ul
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57050
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FlashGel ®回收Starter Kit 含回收Cassettes,回收緩沖液, QuantLadder, Loading Dye, 可視化的玻璃罩和對(duì)照.
Dock需單獨(dú)購(gòu)買
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Each
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5分鐘的DNA回收
Direct DNA recovery using The FlashGel® System for Recovery eliminates agarose gel
preparation, band excision and purification. The system delivers highly efficient recovery,
free from inhibitors and UV-induced damage, in a simple 5-10 minute protocol.
- Go from sample loading to recovery in just 5 minutes
- Recover samples directly from the cassette, without band excision or purification
- Visualize sample recovery at the bench, without UV light Recover at 80% - 100%
efficiency
快速簡(jiǎn)單的程序
1. Load samples in top tier of wells
2. Run until samples reach the second tier of wells (2-5 minutes)
3. Stop the run and add FlashGel® Recovery Buffer
4. Remove the DNA from the wells
The FlashGel® System for Recovery eliminates the need to cut away and then purify bands.
As DNA migrates to the second tier of wells, it is free from the agarose matrix and easily
extracted via pipette, with the aid of the FlashGel® Recovery Buffer. Samples are recovered
at 80% - 100% efficiency, free from inhibitors and ready for subsequent re-amplification, cloning
or other techniques, without additional purification steps.
The FlashGel® System (Cat. No. 57067) contains a FlashGel® Dock, a FlashGel® Camera, a pack
of 1.2% DNA Cassettes, Loading Dye and Marker. To get started with recovery, order the FlashGel®
Recovery Kit (Cat. No. 57050), which contains 1.2% Recovery Cassettes, Recovery Buffer,
QuantLadder, Visualization Glasses and a Control fragment.
許可信息
Some components and technology of the FlashGel® System are sold under licensing agreements.
The nucleic acid stain in this product is manufactured and sold under license from Molecular Probes,
Inc., for use only in research applications or quality control, and is covered by pending and issued
patents. The FlashGel® Dock technology contains Clare Chemical Research, Inc. Dark Reader®
transilluminator technology and is covered under US Patents 6,198,107; 6,512,236; and 6,914,250.
The electrophoresis technology is licensed from Temple University and is covered under US Patent
6,905,585.
閃膠回收系統(tǒng)訂購(gòu)信息
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